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TECHNICAL DATA
SRB LIQUID BROTHUSES SRB liquid broth is a liquid medium in 10 ml sealed sterile vials, suitable for the detection, differentiation and estimation of Sulphate Reducing Bacteria. In industrial systems, Sulphate Reducing Bacteria cause corrosion to the metallic surfaces where they are attached as sessile microbes. The formation of insoluble sulphides, as by product of their metabolism, results in plugging and fouling of the systems they contaminate. The detection and estimation of these bacteria is based in their ability to convert sulphate ion to sulphide which reacts with the ferrous ions to give a black color. Sulphate and ferrous ions are present in the liquid medium, together with the basic ingredients necessary to support the bacterial growth.
RECIPE DESCRIPTIONThe liquid medium is derived from the Postgate Medium F recipe, which in turn is the iron-sulphite agar, widely used in food microbiology, supplemented with lactate and magnesium to support the growth of desulfovibrios and some other desolfotumacula genera (Mara & Williams, 1970). DETECTION OF SRBThe simplest way to use liquid media to estimate the concentration of Sulphate Reducing Bacteria in a water sample is the extinction-dilution technique. A more precise approach is based on the MPN (Most Probable Number) techniques. A detailed procedure for both methods is available on request.
STORAGEStore in a cool place at not more than 25 °C to not reduce the shelf life. SAFETY PRECAUTIONS AND DISPOSALSRB liquid medium is for laboratory use only. Do not inject in humans. Blackened and used vials can be disposed off after autoclave sterilization or chemical sterilization (e.g. inject 1 ml of commercial bleach liquor into each blackened or used vial).
ADDITIONAL INFORMATION CUSTOMS CODE : 38210000
TECHNICAL DATAPHENOL RED LIQUID BROTHUSESPhenol red liquid broth is a 9 ml liquid medium in sealed sterile vials, suitable for the detection, differentiation and estimation of acid producing aerobic bacteria and/or total aerobic bacteria. In industrial systems, acid producing aerobic bacteria cause corrosion and/or fouling over the metallic surfaces where they are attached as sessile microbes. The detection and estimation of these bacteria is based in their ability to cause a change in colour, from red to yellow, of the liquid medium. Phenol red dye, as pH indicator, is present in the liquid medium together with the basic ingredients necessary to support the bacterial growth.
RECIPE DESCRIPTIONThe liquid medium is derived from the classical Phenol red carbohydrate media (DIFCO MANUAL). DETECTION OF AEROBIC BACTERIAInjected vials that turn yellow after incubation indicate the presence of aerobic acid producing bacteria in the sample of tested water. Injected vials that become turbid without change in colour indicate the presence of general aerobic bacteria (not acid producing) in the sample of tested water. The simplest way to use this liquid medium to estimate the concentration of acid producing aerobic bacteria and/or total aerobic bacteria in a water sample is the extinction-dilution technique. A more precise approach is based on the MPN (Most Probable Number) techniques. A detailed procedure for both methods is available on request.
STORAGE Store in a cool place at not more than 25 °C to not reduce the shelf life. SAFETY PRECAUTIONS AND DISPOSALPhenol red liquid medium is for laboratory use only. Do not inject in humans. Used vials can be disposed off after autoclave sterilization or chemical sterilization (e.g. inject 1 ml of commercial bleach liquor into each used vial). ADDITIONAL INFORMATIONCUSTOM CODE : 38210000
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